You can query or browse ReBiT in the following ways:

Browse Functional Groups returns a table containing all 605 structures currently stored within ReBiT.  Highlighting a particular functional group and choosing “Find Enzymes” will open a new window listing the enzymes, other substrates or products and co-factors involved in the reactions that consume or create the structure. 

Browse Names brings up an alphabetical listing of all functional groups in ReBiT.  Selecting a particular name will return a secondary list with more detailed naming and structural information.  Selection of the text string corresponding to a particular structure returns the table of corresponding enzymes.

Query Structure brings up a drawing board that allows one to directly draw a skeletal structure.  After drawing the desired structure, select a search strategy (substructure, superstructure, similarity, exact match, etc.) from the drop-down menu on the left-hand side of the screen.  More stringent search strategies return fewer hits closer to the queried structure, while less stringent searches return more hits consisting of a broader array of structures.  Selecting the search button initiates the query for the drawn structure.

Query SMILES brings up a text box where SMILES code may be input.  After inputting the desired SMILES string, select a search strategy and press the search button to initiate the query. 

After querying ReBiT by structure or SMILES, a list of matching structures will be displayed.  Select the structure of interest and press the “Find Enzymes” button on the menu.  A table of all enzyme-catalyzed reactions involving the selected structure will be shown, along with 3-digit E.C. numbers corresponding to each of the reactions.  In parenthesis below each 3-digit E.C. group is the total number of enzymes in that 3-digit group.  This number serves as an initial assessment of substrate diversity, an important metric for choosing enzymes for new pathways.  Additionally for each enzyme group, graphical representations of all ReBiT structures involved in the canonical reaction are shown as well as any co-factors or co-substrates involved.  Comments (typically suggestions as to what specific 4-digit E.C. numbers catalyze each reaction) are also displayed for each reaction.  Selecting a particular E.C. number directs the user to an ExPASy database page listing all enzymes in the enzyme class selected.  Clicking on a specific enzyme from this list redirects the user to a webpage containing additional information about the selected enzyme class can be found.  Links to other enzyme databases (BRENDA, MetaCyc, KEGG, etc.) may also be found here.
Technical Notes:  The final results table will appear in a new, “pop-up” window.  You must allow pop-ups from this site in order to view these results. This site requires the use of Java. We suggest using the last version from Sun Microsystems.